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Part:BBa_K539691:Experience

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)
Revision as of 15:11, 4 October 2011 by Zcc (Talk | contribs) (→‎Applications of BBa_K539691)

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Applications of BBa_K539691

Butano figure1.jpg

Figure.1 Firstly , we ligase B0030 with ilvC.


Butanol figure2.jpg

Figure.2 At the sametime , we ligase B0030 with ilvD.


Butanol figure3.jpg

Figure.3 Meanwhile , we ligase B0032 with Alss.


Butanol figure4.jpg

Figure.4 To make sure we obtain the correct part , colony PCR is needed.


Butanol figure5.jpg

Figure.5 Secondly , we ligase Plac with B0032+Alss


Butanol figure6.jpg

Figure.6 Thirdly , we ligase Plac+B0032+Alss with B0030+ilvC


Butanol figure7.jpg

Figure.7 At the same time , we ligase B0030+ilvD with RNA thermometer+tetR+double terminator


Butanol figure8.jpg

Figure.8 Finally , we ligase the whole circuit:promoter(lacI regulated)+Alss+ilvC+ilvD(each preceded by own RBS)and RNA thermometer+terminator

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