Reporter

Part:BBa_K598000

Designed by: Yangyang ZHAO, Yan GONG, Zhenrun ZHANG, Qingyang XIAO   Group: iGEM11_Peking_R   (2011-09-18)
Revision as of 13:46, 18 September 2011 by Registry (Talk | contribs)

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TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS+E0040+B0015

This part is TPP up-regulated hammerhead ribozyme, consisting of 131 nucleotides.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 121
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 794


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//plasmidbackbone/copynumber/high
//regulation/negative
Parameters
emission509nm
excitation470nm
ligandsThiamine Pyrophosphate (TPP)
n/aTPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS+E0040+B0015
resistance chloramphenicol