Generator

Part:BBa_K624019

Designed by: Shang-Jui Tsai   Group: iGEM11_NYMU-Taipei   (2011-10-02)
Revision as of 08:32, 2 October 2011 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pYMB essentials+[13-R]+[Y-CHAMP]

pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, 13-R and Y-CHAMP are ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode. This design is made in case EYFP fusion on N-terminal of mms13 has negative effects on its folding.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal NgoMIV site found at 1809
    Illegal NgoMIV site found at 1893
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2559


[edit]
Categories
Parameters
None