![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K524004
Designed by: HO Yuan Heng Trevor Group: iGEM11_HKUST-Hong_Kong (2011-10-03)
pir gene
ori-gamma is part of the replication origin of the R6K origin. Initiation of replication at ori-gamma requires the pi protein in trans, which is encoded by the pir gene. Yet doubling the concentration of pi protein would effectively shut down the replication as well. Expression of pi protein is auto-regulated at its promoter region. Our construct design was to insert a CAP-binding site between the auto-regulating promoter and the CDS, by doing so we can halt replication and induce plasmid loss in nutrient (glucose) rich medium.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 438
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |