Generator

Part:BBa_K649301

Designed by: Goshi Sugano   Group: iGEM11_Tokyo_Tech   (2011-09-19)
Revision as of 04:54, 2 October 2011 by Ratha (Talk | contribs)

Ptrc-RBS-rocF

Arginase is coded with Ptrc and RBS in this part.

rocF is the same as BBa_K649300.
Ptrc and RBS are from pTrc99A.

You should NOT ligate this part into high-copy vector.
In our test, this part ligated into pSB1C3, E. coli DH5α didn't glow after 24 hours incubation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 230
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None