Composite

Part:BBa_K649200:Experience

Designed by: SeoHyun Kim   Group: iGEM11_Tokyo_Tech   (2011-09-18)
Revision as of 13:03, 3 October 2011 by Carakim01 (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K649200

The in vitro assay of BBa_640200 allowed us to confirm that the Cre-mediated recombination on lox2272 cassette works as designed. In the assay, the part was made linear using restriction enzymes. Cre recombinase (1,000 units/ml, 1μl) was added to the linear DNA (50ng/μl, 4 μl) and incubated for 0.5, 2, and 4 hours. Images of the experiments have been added below.

IMAGES

These images of the electrophoresis experiments show that there are several bands in samples to which Cre was added, which indicates that excision of the lox sites successfully occurred.

User Reviews

UNIQ73846a56ff908117-partinfo-00000000-QINU UNIQ73846a56ff908117-partinfo-00000001-QINU