Coding

Part:BBa_K526002

Designed by: Sung Kuk Lee   Group: iGEM11_UNIST_Korea   (2011-09-26)
Revision as of 13:19, 26 September 2011 by Registry (Talk | contribs)

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Temperature dependent Ribo-Switch fused to GFP

We have exploited the temperature dependent mRNA that is associated with the chemotaxis response regulator, CheR, to develop a novel temperature regulated gene expression system. The mRNA is assumed to form a stable secondary structure burrying the RBS and thus blocking gene expression at a higher temperature (37 degree C) where as the temperature is lowered to about 27 degree C the stability of the secondary structure is disturbed thus exposing the RBS and favoring gene expression

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 630
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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