Coding

Part:BBa_K557000:Design

Designed by: Yinghong Lan   Group: iGEM11_USTC-China   (2011-10-02)
Revision as of 12:57, 2 October 2011 by Pythonwawa (Talk | contribs) (→‎Design Notes)

CheZ: phosphatase which dephosphorylates CheY-P and thus plays an important role in E.Coli chemotaxi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We obtained the sequence of CheZ of Top10 in NCBI (NP416395.1) and designed primers to acquire CheZ by PCR.


forward primer:5'-GTTTCGAATTCGCGGCCGCTTCTAGATGCAACCATCAATCAAACCTGCTGACGAGCAT-3'

reverse primer:5'-GTTTCCTGCAGCGGCCGCTACTAGTA-3'

Source

Escherichia coli, the strain has always been kept in the lab of USTC-IGEM

References

Shana Topp, Justin P. Gallivan (2006) Guiding Bacteria with Small Molecules and RNA. J.Am.Chem.Soc, 129,6870-6811