Part:BBa_K672000:Design

Nitrate sensing at low temperatures.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 394
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2298
  

Design Notes

We wanted to make sure that both parts worked in our specific conditions, and for that we did tests in the lab as shown in the experience.

Source

Pyear + RBS + GFP + TT + TT + hyBb + ompA + AOX. It comes from the combination of two other existing Biobricks. For more information consult both other parts: https://parts.igem.org/Part:BBa_K381001 https://parts.igem.org/Part:BBa_K410000

Pyear promoter

• Lin, H.-Y., Bledsoe, P.J., and Stewart, V. 2007. Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen-responsive regulator Fnr in Escherichia coli K-12. J. Bacteriol. 189, 7539-7548

GFP

• See Part:BBa_E0040:Design#References.

Aox

• Grant, N et al. “Two Cys or Not Two Cys? That is the Question; Alternative Oxidase in the Thermogenic Plant sacred Lotus.” Plant Physiology 150 (2009): 987-995. {PAPER}
• HybB Sequence:[[1]]
• OmpA Sequence:http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/e8283dat.Par.0001.File.tmp/e8283dat.pdf
• AoxA Sequence:http://www.ncbi.nlm.nih.gov/nuccore/226897260#sequence_226897260