RBS
Part:BBa_K619892:Design
Designed by: Julianne Grose Group: iGEM11_BYU_Provo (2011-09-27)
mutated prfA thermosensor from listeria
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 18
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 18
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 18
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 18
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Our "wild-type" thermosensor (BBa-19889) Shine-Delagarno has been optimized for expression in E. coli. Otherwise, the "wild-type" sequence is identical to the thermosensor sequence from Listeria monocytogenes.
Source
prfA-UTR from Listeria monocytogenes