Generator

Part:BBa_K511801

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)
Revision as of 04:17, 29 September 2011 by Grantrob (Talk | contribs)

Red Fluorescent Protein Generator (Hef1a-mKate) MammoBlock Device

This MammoBlock composite device produces the monomeric red fluorescent protein mKate at a low, constitutive level driven by the Hef1a promoter.


(1A) This scatter plot shows the distribution of the Hek293 population after it was transfected with the following DNA parts: Hef1a-LacO:eYFP and Hef1a:mKate, both of which were constitutively active, expressing yellow and red fluorescent proteins respectively. We observe a distinct shift of approximately 83% of the population in their eYFP fluorescence (FITC channel), while we observe a 69% shift in mKate fluorescence (PE-TexasRed channel).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal BglII site found at 615
    Illegal XhoI site found at 1014
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
    Illegal NgoMIV site found at 749
    Illegal AgeI site found at 127
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1899
    Illegal SapI.rc site found at 1281


[edit]
Categories
Parameters
None