Regulatory

Part:BBa_K511003

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-25)
Revision as of 23:02, 25 September 2011 by Registry (Talk | contribs)

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UAS-Gal4 Promoter MammoBlock

This part encodes a promoter that is inducible by variants of the Gal4 transactivator and off otherwise.

As shown in Figures 1 and 2, fluorescence from a red fluorescent protein (mKate) driven by the UAS-Gal4 promoter is very strong in the presence of constitutively produced Gal4 derivative with an attached viral VP16 transactivation domain (Gal4-VP16), and is essentially off in the absences of the Gal4-VP16 transactivator.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 126
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 126
    Illegal NheI site found at 154
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 126
    Illegal XhoI site found at 83
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 126
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aUAS-Gal4 Promoter MammoBlock