Conjugation
Part:BBa_K640002:Design
Designed by: Emily Hicks Group: iGEM11_Calgary (2011-09-26)
oriT - Interspecies origin of transfer
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
oriT, or origin of transfer, is a DNA platform which allows for the transfer of plasmid DNA from one organism to another. Relaxases and nickases are responsible for cutting the DNA, rolling circle replication of one of the plasmid strands, and mediated transfer to the receptor organism.