Composite

Part:BBa_K634002

Designed by: Kenneth O. Xu   Group: iGEM11_Wisconsin-Madison   (2011-09-16)
Revision as of 02:48, 29 September 2011 by Ewalters (Talk | contribs)

exaDE

The sensor kinase ExaD responds to the presence of ethanol within the cell and phosphorylates ExaE. The phosphorylated ExaE then acts as an activating transcription factor on PexaA. The operon was studied in Pseudomonas aeruginosa, and found to be under transcriptional regulation by the protein AgmR (1). It was also established that by expressing exaDE under a different promoter, the system could proceed in the absence of AgmR (ibid).

This part is a promoterless monocistronic operon, ready to be put under a desired promoter. When expressed in a cell containing part K634008, genes 5' of K634008 can be expressed in the presence of ethanol.


References

(1) Gliese N., Khodaverdi V., Schobert M., and Gorisch H. (2004) AgmR controls transcription of a regulon with several operons essential for ethanol oxidation in Pseudomonas aeruginosa ATCC 17933. Microbiology 150, 1851-1857.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 158
    Illegal AgeI site found at 305
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None