Coding

Part:BBa_K620000

Designed by: Caltech iGEM 2011   Group: iGEM11_Caltech   (2011-09-20)
Revision as of 23:32, 28 September 2011 by Ashelton (Talk | contribs)

DDT Dehydrochlorinase

Also known as Glutathione S-transferase 1-1, this protein is supposed to degrade DDT, an endocrine disruptor and persistent organic pollutant. The protein was identified from the Anopheles dirus mosquito by [http://www.sciencedirect.com/science/article/pii/0965174895000909 Prapanthadara et al] and the gene was assembled from the protein's amino acid sequence, taken from [http://www.ncbi.nlm.nih.gov/protein/Q93113.1 NCBI's protein database], via PIPE cloning.


Usage and Biology

Plasmid map of K620000 in pSB1C3 (submission plasmid)
Plasmid map of K620000 in pET11-a to amplify and purify the protein.
DDT reaction with DDT Dehydrochlorinase analyzed with GCMS. There is a band at 191g/mole and a band at 206 g/mole
DDT Molecular Structure
DDT structure after GCMS with a molecular weight of 235 g/mole
DDT structure after reaction with DDT Dehydrochlorinase and GCMS with a molecular weight of 206g/mole

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None