Signalling
Aeq

Part:BBa_K548000

Designed by: Peter Qiao   Group: iGEM11_Penn   (2011-09-27)
Revision as of 21:26, 28 September 2011 by Magaraci (Talk | contribs)

Humanized Aequorin

This BioBrick is a human cell line optimized version of Aequorin, a bioluminescent protein found in various jellyfish and other marine organisms. The protein requires Ca2+ for catalytic activity and oxidizes its substrate, coelenterazine to emit blue light (λmax=469nm). Aequorin is NOT a fluorescent protein, it does not require an excitation light source to emit light. Transfection of the gene into 293T cells using lipofectamine has been confirmed. The part contains EcoRI and PstI restrictions sites, and is ligated to the iGEM pSB1C3 backbone. This backbone provides chloramphenicol resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 62
    Illegal NgoMIV site found at 226
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None