Reporter

Part:BBa_K542006

Designed by: Anthony Vuong   Group: iGEM11_Lethbridge   (2011-09-23)
Revision as of 19:47, 23 September 2011 by Anthony.vuong (Talk | contribs)

pBAD Inverse-Regulated Arg-tagged ECFP and EYFP (FRET Reporter)

Made by assembling BBa_K542003 with BBa_K542005.

The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP and Arg-tagged EYFP will be produced constitutively. BBa_K542003 was placed upstream of BBa_K542005, so that expression of the fluorescence proteins maybe "turned off" in the presence of arabinose.

Fluorescence/Förster Resonance Energy Transfer(FRET) is a distance-dependent phenomenon in which the excitation of a donor fluorophore leads to emission by an acceptor fluorophore; this occurs if the two fluorophores are within a certain distance to each other. The distance is dependent on the FRET pair used. The emission spectrum of the donor fluorophore must overlap with the excitation spectrum of the acceptor fluorophore for FRET to occur. FRET is explained in further detail on the [http://2009.igem.org/Team:Lethbridge/Project#FRET Lethbridge 2009 Wiki]. This phenomenon will allow for characterizing co-localization within the Lumazine Synthase microcompartment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None