Composite
CFP
Part:BBa_K566004:Design
Designed by: Daniel Rodriguez Group: iGEM11_UANL_Mty-Mexico (2011-09-26)
CFP protein generator without transcription terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 39
Illegal XhoI site found at 1 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 107
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization for better expression in E. coli. Illegal sites were removed.
Source
Synthesized DNA.
References
1. Patterson G., Rich N. Day and David P.(2001) Fluorescent protein spectra. Cell science at a glance 5:837-838