Composite

Part:BBa_K634007

Designed by: Eric Walters   Group: iGEM11_Wisconsin-Madison   (2011-09-23)
Revision as of 03:51, 27 September 2011 by Ewalters (Talk | contribs)

Double selection cassette (tagRFP-sacB-kanR)

When placed 3' of the promoter portion of a 1- or 2-component sensor, BBa_634007 can be used in the following ways:

Reporter: tagRFP (BBa_K634005) fluoresces red upon induction (see BBa_K634005 for issues with fluorescence)

Selection: Sensor systems can be selected for desired induced expression through supplementation of the culture with the sensor's analyte and kanamycin.

Counter selection: Sensors can also be selected for absence of expression when uninduced through supplementation of the culture with sucrose

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 648
    Illegal SapI.rc site found at 30


[edit]
Categories
Parameters
None