Regulatory
BS

Part:BBa_K566002:Design

Designed by: Daniel Rodriguez, Porfirio Quintero   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)
Revision as of 16:23, 27 September 2011 by Quintero (Talk | contribs) (→‎References)

Biphasic switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 95
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Distance between pRM and OL must be longer than 1.6 kb due to necessity of loop formation for more effiecient repression state of the switch.


Source

Extracted directly from Lambda genome by separate as pRM and OL parts, constructed by classic BioBricks system.

References