Part:BBa_K123003:Experience

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UNIQ141ed3eae207e013-partinfo-00000000-QINU

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jbrown2 ([http://2011.igem.org/Team:Caltech Caltech iGEM team 2011])

We ran the DNA sequence through the ExPASy translator with the following result:

MAGMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAA YEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSP FLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESA KETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQA CRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSTG-RKRRNTRWPATCVR RTCGRLR--SNVLKKTLWRCL-PRTRWFLRCWTRNRRSCTLNTTRPVRSLKRL-WVC-PT WRTVNWFT-STGRNVFRVSLT-PCTTRFTCWNARGWKS--SVWFGVLWNIRVNCCSRRTC CWTVTRVNALKVWLKSSTCCWRPLLVSV--TYKVKSLFA-NLSSC-TLVFTPSCLLP-NL WKKKTTSTVFWTKSPTP-ST-WRKRV-PYNSSTSVWRSCC-SCLTSVTCLTKVWNTCTL- NAKTLFRCTTCCWKCWTRTVCTRRPLVVVRLLKKPTSLTWRPRVLPLLTLYKNTTSPAKR KVSRRPLPV

It looks like there's an intron in the coding sequence. This should be the correct sequence:

>gi|306921463|dbj|BAJ17811.1| estrogen receptor 1 [synthetic construct] MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANA QVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYT VREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFK RSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEV GSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLA DRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG MVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLM AKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASV EETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV

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UNIQ141ed3eae207e013-partinfo-00000002-QINU