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Part:BBa_K560000

Designed by: Zhang Chao   Group: iGEM11_WHU-China   (2011-09-25)
Revision as of 12:49, 25 September 2011 by Registry (Talk | contribs)

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Blue Light Sensor+PtrpL+mRFP1

LovTAP Photosensor is a chimeric protein, which consists of LOV domain which senses blue light. It fused to the trpR-DNA binding domain of the transcription factor trp repressor. Under the presence of blue light, maximum at 450nm, the absorption of a photon leads to conformational rearrangements in LovTAP. The LovTAP can then bind to DNA and repress the transcription of genes following the trpL promoter. Here we use RFP as the reporter.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
    Illegal AgeI site found at 1525
    Illegal AgeI site found at 1637
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 595


[edit]
Categories
Parameters
n/a Blue Light Sensor+PtrpL+mRFP1