Composite

Part:BBa_K539151

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-22)
Revision as of 04:23, 25 September 2011 by Bettyyang (Talk | contribs) (New page: __NOTOC__ <partinfo>BBa_K539151 short</partinfo> This part is a generator of crtEBIY. Promoter BBa_J23103 + CrtEBI(BBa_K274100) is submitted by team Peking 2010. Enzyme cassette CrtEBI (...)

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promoter+crtEBI+RNA thermometer(FourU)+crtY+terminator

This part is a generator of crtEBIY. Promoter BBa_J23103 + CrtEBI(BBa_K274100) is submitted by team Peking 2010. Enzyme cassette CrtEBI (with individual rbs) converts colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). And CrtY converting lycopene to orange beta-carotene. Our team assemble BBa_K346090(promoter+crtEBI) and RNA thermometer+crtY+terminator. This RNA thermometer initiate translation at 37°C. The existence of RNA thermometer makes crtY translated at specific temperature. The terminator is included.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 658
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1735
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None