Composite

Part:BBa_K611010

Designed by: Nicholas Csicsery   Group: iGEM11_UC_Davis   (2011-09-18)
Revision as of 01:16, 25 September 2011 by NCsicsery (Talk | contribs)

LacI Repressor Characterization Construct

This part is used to characterize mutants of BBa_C0012 (LacI). A mutant of BBa_C0012 can be ligated onto the 3' end of the part by cutting this plasmid at SpeI and PstI and by cutting the mutant repressor at XbaI PstI. One can then measure several of the mutant's properties through its regulation of GFP expression. Concentration of the added LacI mutant can be varied through different arabinose concentrations as long as AraC is present.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1216
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1156
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


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