Plasmid

Part:BBa_K530036:Design

Designed by: Daniel Bibl   Group: iGEM11_Johns_Hopkins   (2011-09-24)
Revision as of 22:20, 24 September 2011 by Registry (Talk | contribs)

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pRSBB423 HIS3 2Micron Episomal Yeast Shuttle Vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5258
    Illegal PstI site found at 1183
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1183
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 899
    Illegal BglII site found at 959
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5258
    Illegal PstI site found at 1183
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5258
    Illegal PstI site found at 1183
    Illegal NgoMIV site found at 1748
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3428
    Illegal SapI site found at 2345


Design Notes

QuickChange PCR


Source

pRS 423

References