Plasmid
Part:BBa_K530036:Design
Designed by: Daniel Bibl Group: iGEM11_Johns_Hopkins (2011-09-24)
pRSBB423 HIS3 2Micron Episomal Yeast Shuttle Vector
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 5258
Illegal PstI site found at 1183 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1183
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 899
Illegal BglII site found at 959 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 5258
Illegal PstI site found at 1183 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 5258
Illegal PstI site found at 1183
Illegal NgoMIV site found at 1748 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3428
Illegal SapI site found at 2345
Design Notes
QuickChange PCR
Source
pRS 423