Plasmid

Part:BBa_K530030

Designed by: Daniel Bibl   Group: iGEM11_Johns_Hopkins   (2011-09-24)
Revision as of 00:18, 25 September 2011 by Dbibl1 (Talk | contribs)

pRSBB405 LEU2 Integrating Yeast Shuttle Vector

Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and LEU2 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2799
    Illegal AgeI site found at 1503
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4479
    Illegal SapI site found at 3396


Plasmid Map

PRSBB405.png

[edit]
Categories
Parameters
None