Coding
ADC-AAR

Part:BBa_K590052

Designed by: Austin Moon, Casey Ager, Ingrid Swanson, Seth Sagulo   Group: iGEM11_Washington   (2011-09-15)
Revision as of 21:28, 24 September 2011 by Anjuhye (Talk | contribs) (→‎Usage and Biology)

Aldehyde Decarbonylase to Acyl-ACP Reductase (ADC-AAR) Fusion

This part encodes a direct gene fusion product of the two enzymes, Aldehyde Decarbonylase (ADC) and Acyl-ACP Reductase(AAR), in a linear configuration with a glycine-serine linker for co-localization. For more information, refer to [http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team wiki]

Usage and Biology

We expected this will optimize alkane production developed by the 2011 University of Washington iGEM Team, by bringing ADC and AAR close together. Contrary to what was expected, our gas chromatography-mass spectroscopy (GC-MS) data demonstrated that direct fusion has a negative effect on alkane production.

Washington2011 Alkane Production Localization Graph1.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None