Coding
FabH2
Part:BBa_K590034
Designed by: Matthew Harger Group: iGEM11_Washington (2011-09-15)
FabH2
[http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has some evidence to indicate cause Escherichia coli to produce linear, even-length chain fatty acids.
Usage and Biology
FabH2 is from Bacillus subtilis. The FabH family of proteins initiates fatty acid elongation by converting an Acyl-CoA into an Acyl-ACP, with is extended by 2 carbon units to form longer chain length fatty acids. Normally, FabH proteins use a simple 2-carbon acetyl-CoA to start fatty acid biosynthesis, resulting in linear fatty acids. However, FabH2 can also use Isobutyryl-CoA, Isovaleryl-CoA, and 2-Methylbutyryl-CoA (products from Valine, Leucine, and Isoleucine degredation, resulting in 2-methyl branched fatty acid production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 310
Illegal AgeI site found at 949 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 355
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Categories
Parameters
None |