Regulatory
Supercoilo

Part:BBa_K676003:Design

Designed by: Ejaj Intisar   Group: iGEM11_UCL_London   (2011-09-11)
Revision as of 20:54, 22 September 2011 by Eintisar (Talk | contribs) (→‎Source)

TyrT Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers designed for PCR:

Forward: TAGTTCGAATTCTCTAGACAGTTCATTTTTCTCAACG Reverse: ATCATCCTGCAGACTAGTACGGGGTAATGCTTTTTAC

Final PCR product is 115 bp

Source

Escherichia Coli K-12 genome - from 1286864 to 1286948 bp

References