Coding
mamHI

Part:BBa_K590000

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)
Revision as of 05:31, 22 September 2011 by Saushun (Talk | contribs) (→‎Usage and Biology)

mamHI, from Magnetospirillum magneticum

This part includes gene mamH and mamI, which were extracted from the genome of Magnetospirillum magneticum strain AMB-1 by [http://2011.igem.org/Team:Washington UW iGEM Team 2011].

Usage and Biology

The gene number of mamH is AMB0961; mam I is AMB0962. Specific function of mamH was not reported. But it was found that mamI associates with the magnetosome chain localization, and the loss of mamI causes no membrane formation.

The image below shows the band(right) of HI, which is 1600bp.

1kb ladder(left),HI(right)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 89
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 2589
    Illegal NgoMIV site found at 3920
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 651
    Illegal BsaI site found at 1986
    Illegal BsaI site found at 3317
    Illegal SapI site found at 825
    Illegal SapI site found at 2160
    Illegal SapI site found at 3491


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Categories
Parameters
n/amamHI, from Magnetospirillum magneticum