Coding
Part:BBa_K660201:Design
Designed by: Glasgow Uni 2011 Group: iGEM11_Glasgow (2011-09-16)
Revision as of 04:10, 22 September 2011 by HannahRalph (Talk | contribs)
Latherin (Surfactant Protein) + 6xHIS Tag
Illegal Restriction Sites
We are aware of two illegal restriction sites that exist in our latherin biobrick. Due to time constraints, we were unable to complete site directed mutagenesis. The primers we designed for this are below, for any future team.
1st PstI site:
5': GCG GAA ATT AAA CTG CAA GAT ACC CGT CTG CAA CTG
Forward Primer: 5' CAG TTG CAG ACG GGT ATC TTG CAG TTT AAT TTC CGC
Reverse Primer: 5' GCG GAA ATT AAA CTG CAA GAT ACC CGT CTG CAA CTG
MTP= 79.33
2nd PstI site:
5': GCG ATT GAG CTG CAA AGC GGC AAT CCG C
Forward Primer: 5' G CGG ATT GCC GCT TTG CAG CTC AAT CGC
Reverse Primer: 5' GCG ATT GAG CTG CAA AGC GGC AAT CCG C
MTP= 78.71