Generator
T7 auto-am

Part:BBa_K606036:Experience

Designed by: Axel Séguret   Group: iGEM11_Paris_Bettencourt   (2011-09-18)
Revision as of 04:00, 22 September 2011 by AxelS (Talk | contribs)

T7 emitter RFP + Control in E.coli at 37°C
E.coli at 37°C (trans image)
E.coli at 37°C (rfp image)
E.coli negative control at 37°C (trans image)
E.coli negative control at 37°C (rfp image)


E.coli T7 autoloop without terminator at 37°C (trans image)
File:File:T7 autoloop ctrl-26.jpg
E.coli T7 autoloop without terminator at 37°C (gfp image)
E.coli T7 autoloop with terminator at 37°C (trans image)
E.coli T7 autoloop with terminator at 37°C (gfp image)
E.coli negative control at 37°C (trans image)
E.coli negative control at 37°C (gfp image)

These pictures show that the T7 GFP autoloop system is efficient since some cells are glowing with GFP fluorescence. Thus, we can conclude that the T7 polymerase is activated. Finally, we notice there is no difference between the GFP autoloop with and without terminator before the T7 promoter. It could be due to that terminator which is a B.subtilis terminator. Moreover, we know that this E.coli strain has 4 terminators.