Regulatory

Part:BBa_K515010:Experience

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-06)
Revision as of 17:31, 18 September 2011 by Cs3109 (Talk | contribs) (→‎Applications of BBa_K515010)

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Applications of BBa_K515100

  • The enzymatic reactions of the IAM pathway produce indole-3 acetic acid (IAA) which is an important phytohormone.
  • Many Plant Growth Promoting Rhizobacteria produce IAA
  • Analogues of phytohormones like α-Naphthaleneacetic acid are usually found in rooting powders. Therefore, if we were to express IAA in bacteria in a controlled fashion we could increase root growth.
  • Increased root growth could help maintain top soil.
  • Evidence has shown that using IAA on cotton can increase yields.

User Reviews

We have made a protein gel in order to verify whether the promoter is over-expressing XylE in the BBa_K316005 construct in a DH5-α strain of Escherichia coli. The results showed a clear thick band corresponding to the correct size of the protein.

Protein gel of XylE and control cells. Lane 1 contains the XylE producing E. coli and lane two contains the control cell line. The Ladder is a Fermentas PageRuler.