Measurement
ChiPm-lacZ

Part:BBa_K564013

Designed by: Anna Lewinska   Group: iGEM11_DTU-Denmark   (2011-09-20)
Revision as of 23:50, 21 September 2011 by Lri (Talk | contribs)

Upstream mutated chitoporin part fused with lacZ

ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs. In this biobrick we altered the ribosome binding site for ChiP regulator – ChiX (AAGAGG-->AGGAGA). It was done in order to show that the regulatory system depends on the specific base pairing between ChiP and its regulator. The promoter and reporter have not been altered compared to K564012.

This was verified by β-gal assays as shown in the table below. The measurements are in Miller Units

Strain [http://ecoliwiki.net/colipedia/index.php/NM522 NM522] [http://2011.igem.org/Team:DTU-Denmark/Project_testing_sRNA#Strain_construction IG302]
Genotype chiX + chiX -
PchiP(original):lacZ, BBa_K564012 467 4781
PchiP(mutated):lacZ, BBa_K564013 2353 2403


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None