Coding

Part:BBa_K613021:Design

Designed by: Nadine Guenat   Group: iGEM11_EPF-Lausanne   (2011-09-20)
Revision as of 22:29, 20 September 2011 by Registry (Talk | contribs)

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TetR PQ39YM42LP52 mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mutation was introduced by site-directed mutagenesis


Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced mutations by site-directed mutagenesis. Parts of this mutation had already been described by Helbl and Hillen (1998).

References