Coding
Part:BBa_K613021:Design
Designed by: Nadine Guenat Group: iGEM11_EPF-Lausanne (2011-09-20)
TetR PQ39YM42LP52 mutant
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mutation was introduced by site-directed mutagenesis
Source
The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced mutations by site-directed mutagenesis. Parts of this mutation had already been described by Helbl and Hillen (1998).