Composite

Part:BBa_K607025

Designed by: Olesja Popow   Group: iGEM11_Groningen   (2011-09-18)
Revision as of 22:03, 21 September 2011 by Olesja (Talk | contribs)

PlasB_LasR-LVA_antipBad/araC with 0.6RBS

LasR protein with LVA degradation tag under transcriptional control of PlasB promoter and reverse antisense pBad/araC promoter, the RBS has a strength of 0.6 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
    Illegal NheI site found at 1113
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 645
    Illegal AgeI site found at 842
    Illegal AgeI site found at 1339
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1356


[edit]
Categories
Parameters
None