Other
Part:BBa_K510022
Designed by: David Caballero Group: iGEM11_UPO-Sevilla (2011-09-19)
attTn7
TnsABC+D transposase machinery causes the site-specific insertion of the Tn7 transposon in the attTn7 (from attachment site) sequence, which is found in many bacteria. This insertion site is well conserved in Gram-negative bacteria, an even in higher organisms until humans. However, here we add a portable attTn7 to allow using the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit] in any organism as well as in plasmids.
All the derivatives of the miniTn7 BioBrick toolkit could be inserted in the attTn7 site by transposition.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |