Part:BBa_K091104:Experience

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User Reviews

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• SarahV, KULeuven iGEM team 2011

We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. However, we see a fragment of 1000bp and of ±2000bp.

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