Composite

Part:BBa_K607026

Designed by: Olesja Popow   Group: iGEM11_Groningen   (2011-09-18)
Revision as of 18:27, 18 September 2011 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


PlasB_LasR-LVA_antipBad/araC with 1.0RBS

LasR protein with LVA degradation tag under transcriptional control of PlasB promoter and reverse antisense PBad promoter, the RBS has a strength of 1.0 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
    Illegal NheI site found at 1110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 642
    Illegal AgeI site found at 839
    Illegal AgeI site found at 1336
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1353


[edit]
Categories
Parameters
None