Composite

Part:BBa_K607000

Designed by: Olesja Popow   Group: iGEM11_Groningen   (2011-09-12)
Revision as of 18:13, 21 September 2011 by JoyceM1013 (Talk | contribs)

PhybB_taRNA

taRNA under the control of the hybB promoter. In the AND gate of our system, repression of gene expression is achieved through sequestration of the RBS by formation of a stem loop structure by the 5'-untranslated region of the crRNA. This prevents binding of the RBS by the 30s ribosomal subunit and subsequent translation. The taRNA in this part exhibits partial complementarity to the cis-repressive sequence. Its expression, which is driven by this hybB promoter, allows formation of a RNA duplex molecule and is accompanied by a conformational change in the crRNA structure. The stem-loop structure is unfolded, subsequently abolishing RBS obstruction and allowing translation of the target gene. Reference: Isaacs at al. in 2004

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 399


[edit]
Categories
Parameters
n/aPhybB_taRNA