Coding
LigA

Part:BBa_K525710:Design

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-09-12)
Revision as of 11:55, 12 September 2011 by Registry (Talk | contribs)

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DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1663


Design Notes

PCR


Source

Escherichia coli

References