Signalling
Part:BBa_K546000:Design
Designed by: M. Hesselman Group: iGEM11_Wageningen_UR (2011-09-14)
Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1821
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101
Design Notes
The original R0063 BioBrick part (promoter) of the F2621 BioBrick device (R0063-B0034-C0062-B0010-B0012-R0062) turned out to be absent in this composite BioBrick part, as was shown by a sequence analysis. This K546000 composite BioBrick part now contains an improved malfunctional (version of) the F2621 BioBrick device: K546003.
Source
The lux p promoters and the autoinducer producing enzyme originate from the quorum sensing ability of Vibrio fischeri.