Intermediate
Part:BBa_K568005:Design
Designed by: Team TU Munich 2011 Group: iGEM11_TU_Munich (2011-09-14)
Intermediate optogenetical AND-Gate without T7ptag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 644
Illegal XhoI site found at 1786 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 971
Illegal AgeI site found at 3817 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3820
Design Notes
This part was constructed in order to add T7ptag (BBa_K228000 at 3' site of intermediate construct in a next step.