Composite
Part:BBa_K607000
Designed by: Olesja Popow Group: iGEM11_Groningen (2011-09-12)
Revision as of 18:11, 21 September 2011 by JoyceM1013 (Talk | contribs)
PhybB_taRNA
taRNA under the control of the hybB promoter. In the AND gate, repression of gene expression is achieved through sequestration of the RBS by formation of a stem loop structure by the 5'-untranslated region of the crRNA. This prevents binding of the RBS by the 30s ribosomal subunit and subsequent translation. The taRNA exhibits partial complementarity to the cis-repressive sequence. Its expression, which is driven by a second promoter, allows formation of a RNA duplex molecule and is accompanied by a conformational change in the crRNA structure. The stem-loop structure is unfolded, subsequently abolishing RBS obstruction and allowing translation of the target gene. Reference: Isaacs at al. in 2004
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 399
[edit]
Categories
Parameters
n/a | PhybB_taRNA |