Measurement
LigA

Part:BBa_K525720

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-09-10)
Revision as of 09:38, 17 September 2011 by Adrong (Talk | contribs)

DNA ligase from Escherichia coli (LigA)

NAD+-dependent DNA ligase from E. coli in Freiburg BioBrick Assembly Standard (RFC 25)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1422
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 2020
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1636


[edit]
Categories
//cds/enzyme/ligase
//chassis/prokaryote/ecoli
//proteindomain/internal
Parameters
chassisE. coli
originEscherichia coli