Part:BBa_K584027
Constitutive_Promoter + INP(Ice Nucleating Protein Generator)
This part contains the Constitutive Promoter (J23119) with the Ice Nucleating Protein Generator (BBa_K584024) behind it.
The ice nucleating protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. inaZ is the gene responsible for producing the ice nucleation protein.
We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene inaZ.
To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the inaZ.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 707
Illegal NgoMIV site found at 2219
Illegal NgoMIV site found at 2363
Illegal NgoMIV site found at 2627
Illegal NgoMIV site found at 2771
Illegal NgoMIV site found at 2819
Illegal NgoMIV site found at 2939
Illegal AgeI site found at 478
Illegal AgeI site found at 1883 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 653
Illegal BsaI.rc site found at 3399
Illegal SapI.rc site found at 299
None |