Composite

Part:BBa_K607010

Designed by: Olesja Popow   Group: iGEM11_Groningen   (2011-09-18)
Revision as of 16:16, 18 September 2011 by Olesja (Talk | contribs)

Prm_cI-LVA_antiPlasB with 0.07RBS

cI with LVA-tag under transcriptional control of Prm promoter and reverse antisense lasB promoter, the RBS has a strength of 0.07 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents an auto-inducing loop in which the Prm promoter drives expression of its own inducer cI. The running loop can be turned off by activating the reverse antisense lasB promoter (through LasR protein in the presence of Pseudomonas autoinducer (PAI))

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1125
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aPrm_cI-LVA_antiPlasB with 0.07RBS