Composite

Part:BBa_K565004

Designed by: Juan Ricardo Ortiz Meneses   Group: iGEM11_Valencia   (2011-09-18)
Revision as of 12:00, 18 September 2011 by Registry (Talk | contribs)

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Microcin C51 production device

Microcin C51 is a bacteriocin protein produced by the Escherichia coli pMccC51 plasmid. They make holes in bacterial membranes.

In our project, we used Microcin C51 to kill pathogen cells (in our case they were E. coli DH5alpha strain) by membrane lysis, disinfecting the contaminated water.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4082
    Illegal PstI site found at 759
    Illegal PstI site found at 1239
    Illegal PstI site found at 3088
    Illegal PstI site found at 4334
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4082
    Illegal PstI site found at 759
    Illegal PstI site found at 1239
    Illegal PstI site found at 3088
    Illegal PstI site found at 4334
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4082
    Illegal BglII site found at 1399
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4082
    Illegal PstI site found at 759
    Illegal PstI site found at 1239
    Illegal PstI site found at 3088
    Illegal PstI site found at 4334
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4082
    Illegal PstI site found at 759
    Illegal PstI site found at 1239
    Illegal PstI site found at 3088
    Illegal PstI site found at 4334
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aMicrocin C51 production device