Device
Pbad-ChiXR

Part:BBa_K564011

Designed by: Anna Lewinska   Group: iGEM11_DTU-Denmark   (2011-09-20)
Revision as of 23:31, 20 September 2011 by Registry (Talk | contribs)

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ara-->chiXR (sRNA) device

ChiXR originates from an intercistronic region of the chb operon in Salmonella and E. coli. The transcription of this operon is activated in the presence of the chitooligosaccharides as the genes included in this operon comprises for metabolism of these sugars. ChiXR destabilizes another regulatory small RNA called ChiX by binding to the stem of ChiX transcription terminator hairpin. It is fused with arabinose promoter (Para).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None