Composite

Part:BBa_K568003

Designed by: Team TU Munich 2011   Group: iGEM11_TU_Munich   (2011-09-14)
Revision as of 12:51, 21 September 2011 by AlexH (Talk | contribs)

T7 promoter lacZ reporter part

beta-galactosidase expression upon T7 polymerase binding


Usage and Biology

This part can be used as a reporter plasmid. It expresses beta-galactosidase if there is a T7 polymerase (or a mutant variant) nearby or transcribed through another part. The polymerase binds to the T7 promoter and transcription of the lacZ-Gene can occur, leading to beta-galactosidase expression.


Experimental Testing

The part was tested by the 2011 team of the TU_Munich on the 25th of September 2011. For this purpose, a Miller Assay was conducted. The results can be seen below.

Results of the Miller Assay of BBa_K568003. The part was transcribed by genome-coded T7 polymerase, which was induced by addition of varying concentrations of IPTG. The control strain was not able to produce T7 polymerase.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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